Synthesis of Chlorotoxin by Native Chemical Ligation
نویسندگان
چکیده
منابع مشابه
Protein synthesis by native chemical ligation: expanded scope by using straightforward methodology.
The total chemical synthesis of proteins has great potential for increasing our understanding of the molecular basis of protein function. The introduction of native chemical ligation techniques to join unprotected peptides next to a cysteine residue has greatly facilitated the synthesis of proteins of moderate size. Here, we describe a straightforward methodology that has enabled us to rapidly ...
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A strategy for the synthesis of multivalent peptide-based nonsymmetric dendrimers by native chemical ligation using poly(lysine) dendritic wedges as scaffolds is presented.
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Chemical ligation is a popular and rapidly expanding area of research impacting numerous aspects of organic chemistry, biomolecular and materials science. Native chemical ligation (NCL) in particular, has found numerous applications in such areas. This is likely because NCL is a chemoselective coupling reaction between two, normally peptide, components, one bearing a thioester and the other an ...
متن کاملSelenocysteine in native chemical ligation and expressed protein ligation.
L-Selenocysteine (Sec or U) has been called the “21st amino acid”.1 Like the twenty common amino acids, selenocysteine is inserted during the translation of mRNA and has its own tRNASec and codon, UGA. This codon also serves as the opal stop codon. Decoding a UGA codon as one for selenocysteine requires a special structure in the 3′ untranslated region of the mRNA called a selenocysteine insert...
متن کاملNative chemical ligation in covalent caspase inhibition by p35.
Wide-spectrum caspase inhibition by the baculoviral p35 protein was previously shown to be a consequence of covalent inhibition in which a thioester bond is stably formed between the cleavage residue Asp87 of p35 and the active site Cys360' of caspase-8. Here we show that the N-terminal fragment of cleaved p35 (p35-N) is a circular peptide when dissociated from the caspase. Biochemical and crys...
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ژورنال
عنوان ژورنال: Journal of the Turkish Chemical Society, Section A: Chemistry
سال: 2018
ISSN: 2149-0120
DOI: 10.18596/jotcsa.408517